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Valiant Co Ltd csm ura
Csm Ura, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 165 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/csm+ura/CSM-URA/pmc12965118-148-16-17
Average 95 stars, based on 165 article reviews
csm ura - by Bioz Stars, 2026-10
95/100 stars

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Article Title: A synthetic cell with integrated DNA self-replication and lipid biosynthesis
Article Snippet: The PCR reaction mix consisted of 1× KOD One Master Mix (TOYOBO), 300 nM of each forward and reverse primers (491 and 492 ChD), and ∼0.1 ng μL –1 of plasmid DNA.

Article Title: A Semi‐Quantitative Yeast Complementation Platform for Characterizing Urea and Ammonia Transport by Membrane Channels
Article Snippet: Dissolve in 200 ml Milli‐Q‐purified water: 5.4 g DOB (MP Biomedicals, cat. no. 4025022) 0.154 g CSM‐ura (MP Biomedicals, cat. no. 4511222) For plates : Add 4 g agar (2% [w/v] final; Sigma‐Aldrich, cat. no. 05040) Sterilize by autoclaving or by microwaving (bringing to a boil 2‐3 times) For plates : Allow the solution to cool to hand warm (∼50°C) before pouring (∼25 ml per plate).

Article Title: A transient mutational burst occurs during yeast colony development
Article Snippet: CSM—Ura , MP Biomedicals , 4511222.

Article Title: A Semi‐Quantitative Yeast Complementation Platform for Characterizing Urea and Ammonia Transport by Membrane Channels
Article Snippet: Dissolve in 200 ml Milli‐Q‐purified water: 20 ml 10× YNB stock (see recipe; 1× final) 0.154 g CSM‐ura (MP Biomedicals, cat. no. 4511222) 4 g d ‐galactose (if microwaving medium; 2% [w/v] final; Apollo Scientific, cat. no. BIG1724) Sterilize by autoclaving or by microwaving (bringing to a boil 2‐3 times) Allow to cool If autoclaving : Add galactose after cooling Add 8 ml of 50 mM arginine stock (see recipe; 1 mM final) Store up to 1 month at 4°C

Article Title: Itraconazole resistance in Madurella fahalii linked to a distinct homolog of the gene encoding cytochrome P450 14-α sterol demethylase (CYP51)
Article Snippet: SD-Ura was prepared by combining YNB w/ ammonium sulfate (MP Biomedicals, Santa Ana, CA, USA), CSM-URA (MP Biomedicals), and 2% (w/v) glucose.

Article Title: A synthetic cell with integrated DNA self-replication and lipid biosynthesis.
Article Snippet: Transformation in S. cerevisiae CEN.PK2-1C was carried out using the lithium acetate/single-stranded AR TI CL E IN P RE SS carrier DNA/polyethylene glycol method42.

Transfection:

Article Title: The acyl-CoA synthetase Tg ACS1 allows neutral lipid metabolism and extracellular motility in Toxoplasma gondii through relocation via its peroxisomal targeting sequence (PTS) under low nutrient conditions.
Article Snippet: The isogenic S. cerevisiae strains used in this study (i.e. WT (YB332: MATa ura3 leu2 his3A200 ade2 lys2-801) and faa1∆faa4∆ (YB525: MATa ura3 leu2 his3D200 ade2 lys2– 801 faa1∆::HIS3; faa4∆::LYS2) were kindly provided by Dr. Juliette Jouhet (Commissar iat aux Energies Atomiques et aux Energies Alternatives (CEA), Grenoble, France) and have been described earlier (52, 53). .. After transfection, all the strains described in the present work were grown on synthetic medium containing 6.7 g/L of YNB (MP Biomedicals), 0.77 g/L of CSM-URA (MP Biomedicals) and g/L of glucose, as described. .. In complementation experiments, the media were supplemented with 22.5 mM cerule nin (Sigma-Aldrich), 100 mM FFA, and 0.2% (vol/vol) final concentration of Tween 20 (Sigma-Aldrich).



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